4XEE
Structure of active-like neurotensin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 49.070, 88.110, 161.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.900 |
R-factor | 0.23386 |
Rwork | 0.231 |
R-free | 0.28126 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4grv |
RMSD bond length | 0.006 |
RMSD bond angle | 1.008 |
Data reduction software | XDS (10 Jan 2014) |
Data scaling software | XSCALE (10 Jan 2014) |
Phasing software | PHASER (Version 2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 3.100 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.153 | 0.711 |
Number of reflections | 14628 | |
<I/σ(I)> | 8.9 | 1.5 |
Completeness [%] | 90.6 | 86.7 |
Redundancy | 6.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 16-24% PEG400, 75 mM HEPES, pH 7.0-8.0, 1.7 mM TCEP, 32 mM lithium citrate, 0.9 mM Neurotensin (8-13) |