4XE2
N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.913, 46.630, 59.787 |
| Unit cell angles | 90.00, 107.12, 90.00 |
Refinement procedure
| Resolution | 57.140 - 1.199 |
| R-factor | 0.18611 |
| Rwork | 0.185 |
| R-free | 0.20978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xcj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.220 |
| Data reduction software | iMOSFLM (1.0.4) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.140 | 1.260 |
| High resolution limit [Å] | 1.199 | 1.199 |
| Rmerge | 0.099 | 0.404 |
| Number of reflections | 66281 | |
| <I/σ(I)> | 9.6 | 3.1 |
| Completeness [%] | 96.3 | 94.3 |
| Redundancy | 5.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M HEPES, PEG 3350 |
