4XDM
N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with Geldanamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.400, 44.630, 60.160 |
| Unit cell angles | 90.00, 107.93, 90.00 |
Refinement procedure
| Resolution | 57.240 - 1.500 |
| R-factor | 0.18376 |
| Rwork | 0.182 |
| R-free | 0.22426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xcj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.215 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.240 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.682 |
| Number of reflections | 33584 | |
| <I/σ(I)> | 12.5 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M Hepes, PEG 3350 |
