4XDA

Vibrio cholerae O395 Ribokinase complexed with Ribose, ADP and Sodium ion.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-12
Detector	MAR CCD 130 mm
Wavelength(s)	0.973
Spacegroup name	P 1
Unit cell lengths	59.420, 70.700, 79.880
Unit cell angles	106.29, 97.65, 98.68

Refinement procedure

Resolution	27.420 - 1.750
R-factor	0.1885
Rwork	0.186
R-free	0.22490
RMSD bond length	0.011
RMSD bond angle	1.278
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.420	1.840
High resolution limit [Å]	1.750	1.750
Rmerge		0.617
Number of reflections	117286
<I/σ(I)>	8.4	2.3
Completeness [%]	96.2	94.3
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 6000, MES buffer 0.1mM was used as precipitant. Crystal grown in 7 days.