4XCX
METHYLTRANSFERASE DOMAIN OF SMALL RNA 2'-O-METHYLTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 166.025, 166.025, 74.478 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.980 - 2.840 |
| R-factor | 0.2263 |
| Rwork | 0.225 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jwg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.940 |
| High resolution limit [Å] | 2.840 | 6.110 | 2.840 |
| Rmerge | 0.098 | 0.039 | 0.731 |
| Rmeas | 0.103 | 0.040 | 0.763 |
| Rpim | 0.028 | 0.011 | 0.209 |
| Total number of observations | 180091 | ||
| Number of reflections | 14604 | ||
| <I/σ(I)> | 24.25 | 2.08 | |
| Completeness [%] | 98.4 | 97 | 95.7 |
| Redundancy | 12.3 | 11.5 | 11.7 |
| CC(1/2) | 0.999 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 2.4 M NACL, 0.1 M TRIS-HCL, PH 8.6,4% 1,3-PROPANEDIOL |
