4XCK

Vibrio cholerae O395 Ribokinase complexed with ADP, Ribose and Cesium ion.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-12-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	59.304, 69.553, 78.316
Unit cell angles	106.99, 98.77, 96.46

Refinement procedure

Resolution	26.643 - 2.370
R-factor	0.1914
Rwork	0.188
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rk2
RMSD bond length	0.013
RMSD bond angle	1.278
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.410
High resolution limit [Å]	2.370	2.370
Rmerge	0.070
Number of reflections	47045
<I/σ(I)>	5.1	1.5
Completeness [%]	93.8	93.4
Redundancy	2.1	2.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 6000, 0.1M MES BUFFER, pH 6.0