4XCK
Vibrio cholerae O395 Ribokinase complexed with ADP, Ribose and Cesium ion.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.304, 69.553, 78.316 |
| Unit cell angles | 106.99, 98.77, 96.46 |
Refinement procedure
| Resolution | 26.643 - 2.370 |
| R-factor | 0.1914 |
| Rwork | 0.188 |
| R-free | 0.25070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rk2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.410 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.070 | |
| Number of reflections | 47045 | |
| <I/σ(I)> | 5.1 | 1.5 |
| Completeness [%] | 93.8 | 93.4 |
| Redundancy | 2.1 | 2.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 6000, 0.1M MES BUFFER, pH 6.0 |
