4XC2
Crystal structure of GABARAP in complex with KBTBD6 LIR peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-07-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.893, 57.353, 67.310 |
| Unit cell angles | 65.30, 77.34, 89.95 |
Refinement procedure
| Resolution | 32.730 - 1.900 |
| R-factor | 0.2013 |
| Rwork | 0.198 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d32 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.162 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.750 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.365 |
| Number of reflections | 37496 | |
| <I/σ(I)> | 12.1 | 2.5 |
| Completeness [%] | 92.7 | 91 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Ammonium acetate, 0.01 M Magnesium acetate tetrahydrate, 30% Poly ethylene glycol 8000, 0.1 M Tris-HCl, pH 8.0 |
