4XBQ
Crystal Structure of Human Galectin-7 in Complex with Type 1 N-acetyllactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.085, 55.411, 136.497 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.860 - 2.234 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.811 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.313 |
| High resolution limit [Å] | 2.234 | 2.234 |
| Rmerge | 0.050 | 0.277 |
| Number of reflections | 11562 | |
| <I/σ(I)> | 14.59 | 6.21 |
| Completeness [%] | 98.7 | 96.5 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Hepes pH 7.5, 0.2M Lithium Sulfate, 0.1M Sodium Acetate, 25% (w/v) PEG 4000, temperature 298K |
