4XBP
Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylethanolamine (06:0 PE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-08-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03323 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.426, 111.744, 146.196 |
| Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
| Resolution | 49.159 - 2.940 |
| R-factor | 0.2303 |
| Rwork | 0.228 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fx7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.159 | 3.000 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.594 | |
| Number of reflections | 33714 | |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 92.7 | 94.5 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | Sitting drops contain 4E10 Fab at 10 mg/ml, 10 mM Phosphatidylethanolamine (06:0 PE) sample stock mixed (1:1 ratio) with reservoir solution of 20% PEG 6000, 0.1M Tris, pH 8.5 |
