4XBL
Crystal Structure of Human Galectin-1 in Complex with Type 1 N-acetyllactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.265, 58.211, 111.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.363 - 1.931 |
| R-factor | 0.1993 |
| Rwork | 0.196 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.207 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.056 | 0.246 |
| Number of reflections | 21733 | |
| <I/σ(I)> | 29.67 | 7.1 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris pH 8.0, 0.2M Lithium Sulfate, 30% (w/v) PEG3350, TEMPERATURE 298 K |
