4XA6
Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.546, 46.889, 169.055 |
| Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
| Resolution | 43.887 - 3.420 |
| R-factor | 0.2419 |
| Rwork | 0.239 |
| R-free | 0.29670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no4 1yib |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 15120 | |
| <I/σ(I)> | 14.8 | 4.8 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) |
