4X9X
Biochemical Roles for Conserved Residues in the Bacterial Fatty Acid Binding Protein Family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.343, 41.872, 38.573 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 26.094 - 1.199 |
| R-factor | 0.1189 |
| Rwork | 0.118 |
| R-free | 0.14400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.100 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.357 | |
| Number of reflections | 78877 | |
| <I/σ(I)> | 27.4 | 2.4 |
| Completeness [%] | 94.5 | 77 |
| Redundancy | 4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 0.1 M Citrate pH 5.5, 20% PEG 3000 |
