4X9M
Oxidized L-alpha-Glycerophosphate Oxidase from Mycoplasma pneumoniae with FAD bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 111.590, 111.590, 111.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 2.400 |
| R-factor | 0.15044 |
| Rwork | 0.148 |
| R-free | 0.20376 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dme |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.802 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 18401 | |
| <I/σ(I)> | 22.7 | 4.4 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 23.4 | 23.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 2.68 M NaCl, 2% v/v isopropanol, 0.1 M HEPES pH 7.5 |
