4X9D
High-resolution structure of Hfq from Methanococcus jannaschii in complex with UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.86 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.741, 66.874, 109.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.704 - 1.500 |
| R-factor | 0.1755 |
| Rwork | 0.174 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x9c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.111 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.073 | 0.800 |
| Number of reflections | 68667 | |
| <I/σ(I)> | 17.5 | 2.3 |
| Completeness [%] | 99.5 | 95.7 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | A2 JBCScreen Nuc-Pro 1 (50% PEG200, 0,1M Tris-HCl, pH 8,0) in presence of 25 mM UMP |
