4X8W
dsRBD3 of Loquacious
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-07-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.018, 112.970, 114.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.453 - 2.647 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.25100 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.810 |
High resolution limit [Å] | 2.647 | 2.650 |
Rmerge | 0.035 | |
Number of reflections | 25522 | |
<I/σ(I)> | 33.8 | 6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2% PEG1000 12% PEG8000 50 mM Hepes-NaOH 250 mM NaCl 1mM DTT |