4X8P

Crystal structure of Ash2L SPRY domain in complex with RbBP5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	51.500, 59.100, 70.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.170 - 2.200
R-factor	0.2223
Rwork	0.220
R-free	0.27100
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.600
High resolution limit [Å]	2.200
Number of reflections	11628
<I/σ(I)>	16.5
Completeness [%]	96.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.2 M magnesium chloride hexahydrate, 0.1 M BIS-TRIS, pH 5.5, 25% w/v polyethylene glycol