4X8J
Crystal Structure of murine 12F4 Fab monoclonal antibody against ADAMTS5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 149.080, 149.080, 116.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.2176 |
| Rwork | 0.217 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.945 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.091 | 0.042 | 0.582 |
| Total number of observations | 762232 | ||
| Number of reflections | 55390 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 13.8 | 13 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M Zinc acetate, 20% PEG3350 |
