4X86
Crystal structure of BAG6-Ubl4a complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 62 |
| Unit cell lengths | 72.987, 72.987, 48.481 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.469 - 1.850 |
| R-factor | 0.2075 |
| Rwork | 0.204 |
| R-free | 0.23710 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 |
| Data reduction software | Aimless (0.1.27) |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.480 | 48.480 | 1.890 |
| High resolution limit [Å] | 1.850 | 9.060 | 1.850 |
| Rmerge | 0.045 | 0.027 | 3.491 |
| Rpim | 0.011 | 0.006 | 0.802 |
| Total number of observations | 249572 | 2145 | 15645 |
| Number of reflections | 12717 | ||
| <I/σ(I)> | 31.6 | 109.9 | 1.1 |
| Completeness [%] | 100.0 | 98.9 | 100 |
| Redundancy | 19.6 | 17.9 | 19.8 |
| CC(1/2) | 1.000 | 1.000 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 293 | CAPS-NaOH, AmSO4, LiSO4, 2,2,2-trifluoroethanol, CHAPS |
