4X5G
ecDHFR complexed with folate and NADP+ at 270 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-04-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.721, 59.366, 71.700 |
| Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
| Resolution | 37.830 - 1.900 |
| R-factor | 0.15734 |
| Rwork | 0.155 |
| R-free | 0.20853 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.085 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 25274 |
| <I/σ(I)> | 29.3 |
| Completeness [%] | 91.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 6000, imidazole, calcium chloride |
