4X4P
Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.108, 58.112, 217.806 |
| Unit cell angles | 85.20, 87.98, 89.81 |
Refinement procedure
| Resolution | 29.576 - 3.000 |
| R-factor | 0.2257 |
| Rwork | 0.224 |
| R-free | 0.27380 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.576 | 29.576 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.026 | 0.348 | |
| Rmeas | 0.109 | ||
| Rpim | 0.073 | 0.023 | 0.317 |
| Total number of observations | 107845 | 3255 | 16462 |
| Number of reflections | 53506 | ||
| <I/σ(I)> | 9.1 | 23 | 2.5 |
| Completeness [%] | 94.1 | 91.8 | 96.4 |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 290 | 30% 2-Propanol, 0.1M Sodium Acetate pH 5.3, 0.2M Lithium Sulfate, 4.8% PEG 1.5K |
