4X4M
Structure of FcgammaRI in complex with Fc reveals the importance of glycan recognition for high affinity IgG binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.921, 67.991, 125.028 |
| Unit cell angles | 89.86, 112.25, 89.96 |
Refinement procedure
| Resolution | 40.665 - 3.485 |
| R-factor | 0.2493 |
| Rwork | 0.245 |
| R-free | 0.29570 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.717 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.485 | 3.485 |
| Rmerge | 0.135 | 0.650 |
| Number of reflections | 20950 | |
| <I/σ(I)> | 22.1 | 1 |
| Completeness [%] | 91.5 | 68.8 |
| Redundancy | 5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG8000, 10mM Hepes(pH7.5), 50mM Lithium sulfate |
