4X3N
Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.161, 85.244, 135.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.190 - 1.890 |
R-factor | 0.20643 |
Rwork | 0.204 |
R-free | 0.25237 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.740 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.190 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 72226 | |
<I/σ(I)> | 34.5 | |
Completeness [%] | 98.9 | 88.2 |
Redundancy | 9.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | ammonium acetate, sodium citrate, PEG 4000 |