4X3I

The crystal structure of Arc N-lobe complexed with CAMK2A fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9801
Spacegroup name	P 65
Unit cell lengths	51.527, 51.527, 69.929
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.760 - 1.800
R-factor	0.195
Rwork	0.194
R-free	0.20400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.993
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.763
High resolution limit [Å]	1.800	1.800
Number of reflections	9807
<I/σ(I)>	10.3
Completeness [%]	100.0	100
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2M AMMONIUM SULFATE AND 0.1 M TRIS, PH 8.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K