4X2B
K20A RNA dependent RNA polymerase mutant from Foot-and-Mouth disease Virus complexed with an RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979469 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 94.222, 94.222, 121.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.110 - 2.940 |
| R-factor | 0.21722 |
| Rwork | 0.215 |
| R-free | 0.25858 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u09 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.763 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC (Rigid body refinement) |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 3.090 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.098 | 0.652 |
| Number of reflections | 12228 | |
| <I/σ(I)> | 12.8 | 2.5 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 6.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG 4000, 0.2M MES pH 6.0, 0.1 M Mg(COO3)2 |
