4X29

Structural basis for the enhancement of virulence by entomopoxvirus fusolin and its in vivo crystallization into viral spindles (complex with Zinc)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.93219
Spacegroup name	P 41 21 2
Unit cell lengths	71.790, 71.790, 129.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.310 - 2.405
R-factor	0.1622
Rwork	0.155
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ow5
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BUSTER
Refinement software	BUSTER-TNT (2.10)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.310	30.000	2.491
High resolution limit [Å]	2.405	5.160	2.405
Rmerge	0.119	0.074	0.377
Total number of observations	41467
Number of reflections	13267
<I/σ(I)>	6.21		2.32
Completeness [%]	96.2	92.8	89.19
Redundancy	1.8	3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		301.15	In vivo crystals, grown directly in insects