4X28
Crystal structure of the ChsE4-ChsE5 complex from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.745, 108.095, 82.019 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 46.400 - 1.990 |
| R-factor | 0.15261 |
| Rwork | 0.151 |
| R-free | 0.18615 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.000 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.076 | 0.624 |
| Number of reflections | 109767 | |
| <I/σ(I)> | 19.9 | 2.9 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 25% PEG MME 550, 50 mM MgCl2, 50 mM HEPES sodium pH 7.0 |
