4X1Z

Crystal structure of RHDVb P domain in complex with H type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.984463
Spacegroup name	P 21 21 21
Unit cell lengths	76.170, 76.210, 107.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.997 - 1.360
R-factor	0.109
Rwork	0.108
R-free	0.13240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x1w
RMSD bond length	0.007
RMSD bond angle	1.246
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.066	0.041	0.454
Rmeas	0.068	0.043	0.540
Total number of observations	2048689
Number of reflections	151500	1870	9777
<I/σ(I)>	22.18	51.55	1.89
Completeness [%]	98.3	96.6	86.3
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	Lithium chloride, PEG 6000, Citric Acid