4X1V
Crystal structure of the 2nd SH3 domain from human CD2AP (CMS) in complex with a proline-rich peptide (aa 76-91) from human ARAP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 |
Unit cell lengths | 44.590, 44.590, 32.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.590 - 1.580 |
R-factor | 0.16154 |
Rwork | 0.160 |
R-free | 0.19993 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.057 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.590 |
High resolution limit [Å] | 1.580 |
Number of reflections | 8587 |
<I/σ(I)> | 2.24 |
Completeness [%] | 99.9 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES pH 7.5, 1.4 M tri-sodium citrate dehydrate |