4X1U

The structure of AhpE from Mycobacterium tuberculosis revisited

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	113
Detector technology	CCD
Collection date	2001-01-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000, 0.97937, 0.97918, 0.95370
Spacegroup name	I 4
Unit cell lengths	147.994, 147.994, 33.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 1.870
R-factor	0.1533
Rwork	0.151
R-free	0.18320
RMSD bond length	0.020
RMSD bond angle	1.931
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	46.800
High resolution limit [Å]	1.870
Number of reflections	28807
<I/σ(I)>	1.91
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	1.8M sodium malonate pH 5.0, 0.1M sodium acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K