4X1T
The crystal structure of Arabidopsis thaliana galactolipid synthase MGD1 in complex with UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 63 |
Unit cell lengths | 139.250, 139.250, 38.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.580 - 2.250 |
R-factor | 0.1994 |
Rwork | 0.199 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wyi |
RMSD bond length | 0.007 |
RMSD bond angle | 0.976 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.580 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.042 | 0.532 |
Number of reflections | 20423 | |
<I/σ(I)> | 20.8 | 2.9 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 5.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15-18% ethylene glycol |