4X1O
Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase from Catenulisporales acidiphilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.385, 66.113, 80.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.495 - 1.700 |
R-factor | 0.1808 |
Rwork | 0.177 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mq2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.088 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.072 | |
Number of reflections | 25598 | |
<I/σ(I)> | 32 | 2.2 |
Completeness [%] | 99.0 | 98.4 |
Redundancy | 4.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 0.03 M BisTris, 25.5 % PEG3350 |