4X11
JC Polyomavirus genotype 3 VP1 in complex with GD1a oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.140, 96.100, 127.950 |
| Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.16285 |
| Rwork | 0.161 |
| R-free | 0.20148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nxg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 81125 | |
| <I/σ(I)> | 16.1 | 2.5 |
| Completeness [%] | 98.8 | |
| Redundancy | 5.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% PEG 3350 |
