4X0V
Structure of a GH5 family lichenase from Caldicellulosiruptor sp. F32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-30 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.659, 157.780, 120.803 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 46.650 - 2.798 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.798 |
| Number of reflections | 78399 |
| <I/σ(I)> | 11.5 |
| Completeness [%] | 99.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Li citrate tribasic, 20% PEG 3350 |
