4X01
S. pombe Ctp1 tetramerization domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2012-06-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.071, 156.677, 48.927 |
Unit cell angles | 90.00, 118.55, 90.00 |
Refinement procedure
Resolution | 40.676 - 2.201 |
R-factor | 0.2004 |
Rwork | 0.198 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1T6F |
RMSD bond length | 0.009 |
RMSD bond angle | 0.952 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 32678 |
<I/σ(I)> | 20.7 |
Completeness [%] | 99.9 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Precipitant: 0.2M sodium acetate, 0.1M Tris pH 8.5 16% PEG 4000, with 0.4 uL of 0.1M barium chloride dihydrate additive. Cryoprotection in 25% ethylene glycol. |