4WZF
Crystal structural basis for Rv0315, an immunostimulatory antigen and pseudo beta-1, 3-glucanase of Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.133, 65.847, 67.449 |
Unit cell angles | 90.00, 105.17, 90.00 |
Refinement procedure
Resolution | 24.783 - 1.699 |
R-factor | 0.1831 |
Rwork | 0.182 |
R-free | 0.21120 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.171 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.699 | 1.699 |
Rmerge | 0.136 | 0.228 |
Number of reflections | 62875 | |
<I/σ(I)> | 9.51 | 7.13 |
Completeness [%] | 98.4 | 99.9 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M Ammonium phosphate monobasic, 20% PEG 3350 |