4WYW
Mutant K20E of 3D polymerase from Foot-and-Mouth Disease Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.700, 91.700, 118.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.800 |
| R-factor | 0.20411 |
| Rwork | 0.203 |
| R-free | 0.22663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u09 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.577 |
| Number of reflections | 47356 | |
| <I/σ(I)> | 24.15 | 3.5 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG4000, 0.2M Mg(CH3COO)2, 0.1M MES pH6.0, 4% gamma-butyrolactone |
