4WYT

Crystal Structure of Scribble PDZ34 tandem at 2.6 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	69.140, 70.850, 92.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.658 - 2.600
R-factor	0.2283
Rwork	0.226
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tp3
RMSD bond length	0.002
RMSD bond angle	0.521
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.483	46.375	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.041	0.575
Rmeas	0.136
Rpim	0.055	0.020	0.255
Total number of observations	44038	1323	6451
Number of reflections	7301
<I/σ(I)>	10.2	15.4	3.9
Completeness [%]	100.0	99	100
Redundancy	6	5	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	3.0 M sodium chloride, 0.1M Tris-Hcl