4WYI
The crystal structure of Arabidopsis thaliana galactolipid synthase, MGD1 (apo-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9811 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.610, 65.619, 80.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.810 - 2.500 |
| R-factor | 0.1815 |
| Rwork | 0.181 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11358 | |
| <I/σ(I)> | 14.3 | 4.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M magnesium chloride, 22% PEG 3350 |
