4WYH

Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-20
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	28.258, 36.232, 83.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.424 - 1.950
R-factor	0.1598
Rwork	0.158
R-free	0.18010
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.733
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	23.424
High resolution limit [Å]	1.950
Number of reflections	6218
<I/σ(I)>	105.56
Completeness [%]	92.8
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.2 M potassium iodide, 20% (w/v) polyethylene glycol 3,350, pH 7.0