4WY9
Crystal structure of the periplasmic sensory domain of the Campylobacter jejuni chemoreceptor Tlp1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 39.260, 43.320, 50.900 |
Unit cell angles | 92.52, 111.38, 114.73 |
Refinement procedure
Resolution | 15.000 - 1.400 |
R-factor | 0.1187 |
Rwork | 0.117 |
R-free | 0.15420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TlpC |
RMSD bond length | 0.014 |
RMSD bond angle | 1.308 |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.270 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.031 | 0.149 |
Rpim | 0.031 | 0.149 |
Total number of observations | 97980 | 3448 |
Number of reflections | 49677 | |
<I/σ(I)> | 11.9 | 3.9 |
Completeness [%] | 91.5 | 66.2 |
Redundancy | 2 | 2 |
CC(1/2) | 0.998 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 200 mM ammonium acetate, 23%(w/v) PEG 3350 and 100 mM Bis-Tris pH 5.5 |