4WXW

SadC (323-487) from Pseudomonas aeruginosa PAO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	50.913, 51.102, 54.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.295 - 1.800
R-factor	0.1863
Rwork	0.185
R-free	0.21700
RMSD bond length	0.007
RMSD bond angle	1.097
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	13694
<I/σ(I)>	36.2
Completeness [%]	99.1
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M Tris-HCl, 0.2 M MgCl, 26% PEG3350, 25 mM nonanoyl-N-methylglucamide