4WXU

Crystal Structure of the Selenomthionine Incorporated Myocilin Olfactomedin Domain E396D Variant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2013-10-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.484, 50.560, 50.537
Unit cell angles	90.00, 97.27, 90.00

Refinement procedure

Resolution	19.598 - 2.092
R-factor	0.1852
Rwork	0.178
R-free	0.20140
RMSD bond length	0.003
RMSD bond angle	0.830
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.600
High resolution limit [Å]	2.092
Number of reflections	14363
<I/σ(I)>	25.23
Completeness [%]	97.2
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350 Bis-Tris pH 6 magnesium formate