4WXQ

Crystal Structure of the Myocilin Olfactomedin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2010-10-31
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	I 2 2 2
Unit cell lengths	68.660, 85.820, 88.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.779 - 2.150
R-factor	0.1971
Rwork	0.193
R-free	0.23060
RMSD bond length	0.002
RMSD bond angle	0.625
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.780
High resolution limit [Å]	2.150
Number of reflections	14521
<I/σ(I)>	15.54
Completeness [%]	99.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Peg 3000 Peg 2000 MES pH 6.0