4WXK
Crystal structure of a peptide deformylase from Haemophilus influenzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-05-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.036, 80.961, 98.586 |
Unit cell angles | 90.00, 97.81, 90.00 |
Refinement procedure
Resolution | 25.370 - 2.050 |
R-factor | 0.16382 |
Rwork | 0.161 |
R-free | 0.22161 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g2a |
RMSD bond length | 0.017 |
RMSD bond angle | 1.828 |
Data reduction software | HKL-3000 |
Data scaling software | DENZO |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.370 |
High resolution limit [Å] | 2.050 |
Number of reflections | 39520 |
<I/σ(I)> | 11.67 |
Completeness [%] | 97.7 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 20-25% PEG 3350, 0.1M HEPES pH 7.0, 2% glycerol |