4WXJ
Drosophila muscle GluRIIB complex with glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.314, 94.527, 119.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.054 - 2.002 |
R-factor | 0.1993 |
Rwork | 0.198 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s50 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.647 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1819)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.069 | 0.840 |
Number of reflections | 40664 | |
<I/σ(I)> | 27.6 | 2.3 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.5 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Reservoir: 18% PEG 2K MME, 0.1 M Tris pH 8.0, 5 mM NiCl2 Protein buffer: 150 mM NaCl, 10 mM Tris pH 8.0, 2 mM Na glutamate, 1 mM EDTA multiple rounds of seeding |