4WXB
Crystal Structure of Serine Hydroxymethyltransferase from Streptococcus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-04-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.970 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.104, 113.431, 133.388 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 66.540 - 2.050 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kkj |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.972 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.100 | 2.150 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.101 | 0.514 |
| Number of reflections | 187621 | 15884 |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 8 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES 20 mM, pH 7,5 Sodium citrate 0.8-1.2 M Cacodylate 1 M, pH 6,5 |
