4WWX

Crystal structure of the core RAG1/2 recombinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	168.760, 180.050, 200.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.742 - 3.200
R-factor	0.2077
Rwork	0.205
R-free	0.25910
RMSD bond length	0.007
RMSD bond angle	1.114
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	49907
<I/σ(I)>	12.75
Completeness [%]	98.8
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MES (pH 7.1), 10-15% PEG 3350, 200 mM tribasic ammonium citrate (pH 7.0), 100 mM KCl