4WWR

Crystal Structure of Bag6-Ubl4A Dimerization Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	47.235, 56.494, 75.545
Unit cell angles	90.00, 96.23, 90.00

Refinement procedure

Resolution	19.647 - 2.000
R-factor	0.2267
Rwork	0.225
R-free	0.26880
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.072
Data scaling software	Aimless (0.3.6)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.650	19.650	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.047	0.013	0.813
Rpim	0.033	0.010	0.565
Total number of observations	78055	818	5819
Number of reflections	26499
<I/σ(I)>	13.9	49.6	1.7
Completeness [%]	98.6	88.7	98.2
Redundancy	2.9	2.8	3
CC(1/2)	0.999	0.999	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298.13	20% PEG-3350, 0.05 HEPES sodium pH 7.0, 1% tryptone