4WW7

Crystal structure of binary complex Bud32-Cgi121 in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	I 41
Unit cell lengths	113.890, 113.890, 87.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.015 - 1.669
R-factor	0.1943
Rwork	0.193
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Methanocaldococcus jannaschii Bud32/Cgi121
RMSD bond length	0.008
RMSD bond angle	1.093
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.020	1.729
High resolution limit [Å]	1.669	1.669
Number of reflections	64292
<I/σ(I)>	18.48
Completeness [%]	99.4	98.46
Redundancy	3.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	0.1 M Sodium Acetate pH 4.8, 2.0 M Ammonium Sulfate, 0.1 M NaCl, 10 mM Tris-HcL pH 7.5, 5 mM AMP and MgCl2