4WW7
Crystal structure of binary complex Bud32-Cgi121 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | I 41 |
| Unit cell lengths | 113.890, 113.890, 87.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.015 - 1.669 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Methanocaldococcus jannaschii Bud32/Cgi121 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.093 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.020 | 1.729 |
| High resolution limit [Å] | 1.669 | 1.669 |
| Number of reflections | 64292 | |
| <I/σ(I)> | 18.48 | |
| Completeness [%] | 99.4 | 98.46 |
| Redundancy | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1 M Sodium Acetate pH 4.8, 2.0 M Ammonium Sulfate, 0.1 M NaCl, 10 mM Tris-HcL pH 7.5, 5 mM AMP and MgCl2 |
