4WW5

Crystal structure of binary complex Bud32-Cgi121 in complex with AMPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-04
Detector	PSI PILATUS 6M
Wavelength(s)	0.980110
Spacegroup name	I 41
Unit cell lengths	112.930, 112.930, 87.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.927 - 1.997
R-factor	0.1853
Rwork	0.183
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.083
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.930	2.069
High resolution limit [Å]	1.997	1.997
Number of reflections	37119
<I/σ(I)>	27.29	5.54
Completeness [%]	99.8	98.1
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Sodium Acetate pH 4.6, 2.0 M Ammounium Sulfate, 0.1 M NaCl,10 mM Tris-HCl pH 7.5, 5 mM AMPPNP and 5 mM L-threonine.